タイトル無し

概要

論文の詳細を見る
The amount of glycogen and the activities of phosphorylase and UDPG-glycogen transferase were histochemically examined in Mongolian gerbil eggs throughout the stages from 1-cell to the blasto-cyst. The eggs were collected under following conditions: 7 h after ovulation for 1-cell eggs, 31 h for 2-cell eggs, 79 h for 4-cell eggs, 103 h for 8-cell eggs, 127 h for morulae, and 151 h for blasto-cysts. For the demonstration of glycogen, eggs were treated with the periodic acid-Schiff with or without a preceding salivary test. The activity of phosphorylase was demonstrated by a modified TAKEUCHI and KURIAKI method and UDPG-glycogen transferase by the TAKEUCHI and GLENNER method.<BR>Glycogen granules were abundant in the eggs throughout the stages from 1-cell to 8-cell, but slight in the morula and blastocyst. Phosphorylase activity was moderate in the 1-cell eggs, weak at the stages from 2-cell to 8-cell, but none in the morula and blastocyst. UDPG-glycogen trans-ferase activity was dot demonstrated in the eggs at any developmental stage.<BR>From the results obtained here, Mongolian gerbil eggs seem to metabolize glycogen at the stages from 1-cell to 8-cell, but do not at the stages of morula and blastocyst. In other hand, the eggs are unlikely to synthesize glycogen throughout their developmental stages.
日本繁殖生物学会の論文