Studies on deep-freezing of boar semen:VI. Effects of rapid freezing on survival of boar spermatozoa

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概要

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• Recently several reports have indicated that the rapid freezing methods gave fairly good results on livability of bull spermatozoa. The present study was made to investigate various rapid freezing rates from several temperature levels for boar spermatozoa. Semen was collected artificially from three boars and its sperm rich fraction was used for this experiment. Semen was centrifuged at 1500 r.p.m. and separated into sperm and seminal plasma. Sperm portion was resuspended with S-G-S diluent<SUP>6)</SUP> to give 2×10<SUP>8</SUP> total spermatozoa per milliliter of diluted semen. After the semen was cooled down slowly to 10°C, it was extended with S-G-S diluent containing 14% glycerol. Glycerol equilibration was taken place for one hour. The semen was sealed in 1 m<I>l</I> glass ampules. The alcohol bath of control was cooled at the rates of 1°C/min from 10°C to -10°C, 3°C/min from -10°C to -25°C and 5°C/min from -25°C to -79°C. When the temperature of the cooling alcohol bath reached at -5, -10, -20, -30 and -40°C respectively, the experimental samples were transferred directly to the alcohol bath which was kept at -80°C. The samples were thawed in a water bath which was kept at 10°C. <BR>The percentage of live sperm were microscopically estimated at 38°C immediately after thawing (Table 1). <BR>The mean percentage of live spermatozoa decreased with the following order regardless the storage periods; <BR>control>-40>-30>-20>-10>-5°C.<BR>There were statistically significant (P<0.01) differences among these groups (Table 2 and Figure 1). <BR>The changes of the temperature of the samples were recorded by the thermoelectric thermometer (Figure 2). <BR>The results indicated that the rapid cooling down to -80°C from the temperature range between -5°C and -30°C was detrimental to the livability of boar spermatozoa.

• 日本繁殖生物学会の論文

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