Immunological studies of gonadotrophins in animal. II. Some biological properties of antiserum to bovine luteinizing hormone.
スポンサーリンク
概要
- 論文の詳細を見る
Some serological characteristics of an anti bovine luteinizing hormone (NIH-LH-B<SUB>3</SUB>) rabbit serum were reported in the previous paper and it was concluded that the serologically specific antiserum to LH was obtained after absorption with bovine serum and bovine thyroid stimulating hormone. <BR>In the present paper some biological properties of an anti bovine LH serum were reported. <BR>The antiserum was obtained by immunizing rabbits with bovine LH by the modified method of Moudgal and Li<SUP>16)</SUP>. <BR>The effect of the antiserum on the biological activities of bovine and ovine LH were studied by the method of rat ovarian ascorbic acid depletion described by Parlow<SUP>11)</SUP>. A group of rats was injected LH and the antiserum while the other, control group, was injected LH and normal rabbit serum. Ovarian ascorbic acid depletion of the rats injected LH and the antiserum was less than that of control. Therefore, it became obvious that biological activities of bovine and ovine LH were inactivated by the antiserum to bovine LH. <BR>The effects of the antiserum on the biological activities of bovine, ovine and porcine gonadotrophins (GTH) were determined by the rabbit ovulation test. Ovulation occured in the control rabbits injected intravenously mixture of rabbit serum and GTH of the each species, but did not appear in the rabbits injected mixture of the antiserum and GTH of the each species. The rasults show that the biological activities of bovine, ovine and porcine GTH were inhibited by the antiserum. <BR>The effects of the antiserum on the biological activities of PMSG and HCG were determined by the rat ovarian weight method. The ovarian weight of rats injected PMSG and the antiserum was less than that of control group. This shows that the biological activities of PMSG was inhibited by the antiserum. As to HCG, however, the ovarian weight of rats injected HCG and the antiserum was not reduced than that of control group. This shows that the biological activities of HCG was not inhibited by the antiserum. <BR>The effects of the antiserum on the biological activities of endogenous GTH in rabbt and rat were examined. Some adult female rabbits were injected the antiserum intravenously and immediately after injection they were mated with male rabbits. Control rabbits were injected a normal rabbit serum instead of the antiserum. None of the rabbits injected the antiserum had ovulation but all of the control rabbits had ovulation. Some intact male rats, 22 days old, were injected the antiserum intrave-nously for 5 days. On the 27th day of birth, they were autopsied and the weights of testis and ventral prostate were measured. The weights of testis and ventral prostate of rats treated with the antiserum were less than that of control rats which received the injection of normal rabbit serum. These results show that the antiserum inhibited the biological activities of endogenous GTH in the rabbit and the rat.
- 日本繁殖生物学会の論文
日本繁殖生物学会 | 論文
- 近交系Hatanoラットに認められた春機発動時期および血漿中ホルモン濃度の系統差
- ラット卵子の自発的第一卵割に及ぼすCa2+/Mg2+不含培養液の影響
- 体外受精由来ウシ胚盤胞のガラス化保存においてバイオプシー後の培養時間が融解後の生存性に与える影響
- ブタ生体回収由来ガラス化胚の生存性の改善
- 卵細胞質内注入法を用いた牛の核移植