Effects of glycerol and phosphoglycerol on the respiration and motility of spermatozoa in domestic animals

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Effects of glycerol and phosphoglycerol on the respiration and motility of spermatozoa have beee studied with different species of domestic animals. Bull, stallion, boar, ram, goat and rabbit semen were collected by means of artificial vagina. Respiration of washed spermatozoa was measured in Warburg manometer at 37°C in the presence of glycerol (0.01 M) and, α and β phosphoglycerol (0.01 or 0.02 M). After 3 hrs of the incubation, motility and glycerol consumption of spermatozoa were examined.<BR>Glycerol increased the rate of oxygen uptake and motility of bull and ram spermatozoa. These effects were low in goat and boar spermatozoa, and almost nil in stallion and rabbit spermatozoa. Glycerol consumption by spermatozoa was higher in bull and ram than in goat, and little in boar.<BR>Phosphoglycerol (α or/and β) increased the rate of oxygen uptake of spermatozoa in all the species examined. On the whole, phosphoglycerol more increased the sperm motility than did glycerol, although the effect of the former was obscure in stallion and rabbit.<BR>The cause of the species differences in glycerol and phosphoglycerol utilization by the spermatozoa was discussed in relation to the pathway of glycerol metabolism and the enzymic activity in the cells.
日本繁殖生物学会の論文