Optimal dose of HCG to induce the ovulation of the immature mouse pretreated with PMS
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The ovulation by the supplemental administration of LH (HCG) in the immature animal pretreated with PMS is being adopted as a new biological assaying for gonadotropic hormones. This technic shows a high sensitivity compared with the former methods judging from the increase in weight of sex glands in the test animal. However, there remain some problems, such as age or size of animal, dosage of hormone, etc., to accomplish its procedure as an universal method. The present study was undertaken to determine the optimal dose of PMS and HCG inducing the ovulation in the immature mouse.<BR>Approximately 335 female mice weighing 8 to 10g at 20 to 22 days of age were used. They are Kasukabe strain which has been kept at the National Institute of Animal Industry. The PMS (Tomoda Pharm. Co. Ltd.) dissolved in physiological saline was injected subcutaneously at the abdominal part of the animal. The animals pretreated with various doses of PMS were injected intraperitonealy with HCG (Tomada Pharm. Co. Ltd.) 54 hours later. Then the animals were killed at 20 hours after the injection of HCG. The number of ova released and percentage of ovulating mice were inspected. At the same time, ovary and uterus were weighed with the torsion balance.<BR>1. The supplemental injection of 0.5 to 0.9I.U. of HCG in the mouse pretreated with a little dose of 0.5I.U. of PMS induced a 100% ovulating response. The treatment of 0.1 to 0.9I.U. of HCG to the priming dose of 4.0I.U. of PMS caused a 100% ovulation in all lots (Table 1). There appeared a regression curve as shown in Fig. 1 between amounts of PMS and percentage of mice ovulating. The injection of dosages over 4.0I.U. of PMS brought about a harmful response to the ovulation.<BR>2. Relation between number of ova released and does of the hormone injected has a close resemblance to that shown in the regression curve in the percentage of ovulation (Fig. 2). The assaying by the response based on the number of ova released may be superior to that by the percentageof mice ovulating. But it is in need of more detailed examination.<BR>3. Considering these two ovulating responses as mentioned above the supplemental injection of 0.3 to 0.5I.U. of HCG in the immature mouse pretreated with 3.0I.U. of PMS will induce the reasonable ovulating result. However, it is desirable to use the suitable amount of PMS for priming in an actual assaying of gonadotropic substance.<BR>4. It is important to choose the uniform animal in size and sensitivity for assaying of gonadotropic hormones.
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