Interactions of cadmium with yeast mannans.
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The interactions of cadmium (Cd) ions with mannans from six species of yeast were studied using equilibrium dialysis. Among the six mannans examined, the binding capacity of the mannan of S. carlsbergensis was the greatest, at about 146 μmol/g mannan. For the other mannans, the amounts of bound metal ions ranged from 20-46 μmol/g annan. However, the dissociation constants of Cd-binding ot each mannan were very similar, at about 0.5 × 10<SUP>-3</SUP> M. Scatchard plot analysis indicated that the mannans had a single type of binding sites.Pronase digestion of the mannans decreased their total nitrogen, accompanied with significant losses of Cd-binding. These results suggest that the carbohydrate moieties of the mannans were not positively involved in the Cd-binding of the yeast cell walls.
- 社団法人 日本薬学会の論文
社団法人 日本薬学会 | 論文
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