A new urine-cytology fixation-method developed at Kure Mutual Aid Hospital.:Kure Mutual Aid Hospital
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概要
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We have developed a new fixation-method for urine cytology. It consists of two sequential steps using 2 fixation cocktails. The first fixation-cocktail consists of 0.2% sodium azide and 50% etanol containing 0.8% EDTA-3 K. The second cocktail consists of 2% polyetylene glycol and 95% isopropyl alcohol. In the first step, urine is mixed with 20 m<I>l</I> of the first fixation-cocktail in a 50m<I>l</I>-sized plastic tube and stored for more than one hour at room temperature. After two centrifugations, several droplets of the second fixation-cocktail are added to the urine specimens for the second step. Then the specimens are smeared on silan-treated glass slides. Advantages to this method are follows: 1) this method is simple and needs no special instruments. 2) it makess it possible to smear almost all of the centrifugated cells on to the glass slides. 3) high concentration of cells leads to high efficacy of cytoscreening, 4) red cells are well preserved, 5) no bacterial proliferation follows, 6) diminidiminished crystal formation in the specimens is allows for better microscopic observation, and 7) the method allows the specimens to be stored after the first step for about one week with few cellular changes. This method is very useful for routine urine cytology work and also beneficial for urine checkups.
- 特定非営利活動法人 日本臨床細胞学会の論文
特定非営利活動法人 日本臨床細胞学会 | 論文
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