Studies of cell ball-like clusters in peritoneal washings obtained at operation using an immunoperoxidase technique.
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Utilizing immunoperoxidase techniques, we evaluated "cell ball" -like clusters ("cell balls"), the origin of which is sometimes difficult to determine. This paper presents the cytological features of "cell balls" detected in 32 (18.4%) of 174 peritoneal washings. The cellular configuration consisted of oval cell clusters forming solid masses surrounded by squamoid cells. The interior of the "cell balls" stained deep blue with Papanicolaou's stain, which demonstrated the presence of homogeneous and amorphous materials, and sometimes showed a fingerprint-like staining pattern. Immuno-cytochemically, almost all "cell balls" were positive for cytokeratin and vimentin. However, the inner material stained positive with antibodies against collagen types I and III. Therefore, these "cell balls" are apparently serous membrane fragments composed of mesothelial cells and fibrous tissue. These findings suggest that immunocytochemical study is a useful method of characterizing "cell balls" in peritoneal washings, which exhibit features similar to those of primary and metastatic tumors and may thus complicate cytodiagnosis.
- 特定非営利活動法人 日本臨床細胞学会の論文
特定非営利活動法人 日本臨床細胞学会 | 論文
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