Observation on the Host Reaction to the Cultivated M. lepraemurium in the Spread Subcutaneous Tissue Preparations of Mice
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概要
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Cultivated murine leprosy bacilli from rough colonies MHO-R_??_ and smooth colony variant (HO-S) were subcutaneously inoculated into mice of inbred strains (C3H, C57BL/6, BALB/c and DDD). Growth patterns of the bacilli of each strain and cellular reactions of host in subcutaneous tissue at the inoculation site were examined by means of the spread tissue preparation technique.There were no remarkable differences in cellular reactions at the inoculation site among these inbred strains of mice infected with HO-R bacilli 2 days and 1 week after the infection. An acute inflammatory reaction with accumulation of many polymorphonuclears was observed at 2 days. At one week, it was disappeared and monocytes and macrophages were dominant. A vigorous granulomatous reaction consisted of polymorphonucleares and lymphocytes developed in C57BL/6, BALB/c and DDD mice at 3 weeks. In the course of the following weeks the cellular reaction was augmented in C57BL/6 and BALB/c mice and further local spread of the infection was arrested. On the other hand, in DDD mice the diminution of cellular reaction occured at 8 weeks and bacilli increased in number. In contrast, no signs of host reactions were observed in C3H mice and many murine leprosy cells were observed at the inoculation site.C57BL/6 and BALB/c mice infected with HO-S bacilli showed essentially the same host reaction as those observed in the experiment with HO-R bacilli. The diminution of cellular reaction observed in the DDD mice infected with HO-R bacilli could not be found in those infected with HO-S bacilli and the multiplication of the bacilli was not seen. In C3H mice infected with HO-S bacilli no specific host reaction was found untill 5 weeks, but cellular reactions happened at 7 to 8 weeks which were never observed in C3H mice infected with HO-R or H-M bacilli. The bacilli decreased in number from the 8th to the 10th week but bacillary multiplication was gradually recovered at 15 weeks. The multiplication of HO-S bacilli was very slow even in C3H mice throughout the observation period.In this study, we found that slower development of mouse leprosy with HO-S bacilli in C3H mice is due to their poor ability to multiply in vivo and the inexplicable host reaction.The mouse strain differences in response to cultivated M. lepraemurium were also discussed on the bases of cell-mediated immunity in the host.
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