A Novel Method for Collecting Ovulated Eggs Using a Liquid Pump in Barfin Flounder, Verasper moseri, and Spotted Halibut, V. variegatus

概要

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Maintaining a high genetic variability in fertilized eggs through the avoidance of the loss of facility-held mature females is fundamental importance for artificial insemination in barfin flounder, <I>Verasper moseri</I>, and spotted halibut, <I>V. variegatus</I>, which are potentially endangered species. The objective of the present study is to establish a new method for collecting ovulated eggs without causing harm and minimizing stress to females, instead of the hand-stripping, using peristaltic pumps with artificial ovarian cavity fluid (AOCF) . By pumping about one liter of sterile AOCF though a vinyl tube which was inserted into an upperpart of the ovarian cavity from the urogenital pore, ovulated eggs were efficiently extruded with the introduced AOCF When ovulated eggs were preserved in AOCF at 4°C, high fertilizability was maintained during 4 and 24 hours in eggs of barfin flounder and spotted halibut, respectively. The developed technique, coupled with our previously developed short-term sperm preservation technique, will enable more controlled conditions for artificial fertilization. The present results demonstrated the practical usefulness of the newly developed method for a convenient and minimally-damaging method of collecting eggs from barfin flounder and spotted halibut.