Isolation and Identification of Antimutagen from MOMIJIGASA (Cacalia delphiniifolia)

概要

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The antimutagenic activity of the extracts of MOMIJIGASA (Cacalia delphiniifolia) was assayed using the Ames test with Salmonella typhimurium TA98 and the mutagen of Trp-P-1 (3-Amino-1, 4-dimetyl-5H-pyrido [4, 3-b] indole). The activity was detected in some of the fractions extracted with Et2O, EtOAc and n-BuOH, suggesting the existence of several active components in MOMIJIGASA. The fraction extracted with EtOAc, which showed the strongest antimutagenic activity, was separated into 4 fractions by silica gel column chromatography.The third, most abundant fraction with the strongest activity was subjected to reversed phase HPLC with a preparative ODS column that gave 5 separated peaks in the chromatogram.The HNMR spectrum of the third, most abundant peak showed the presence of caffeic acid and quinic acid moieties with the signal-intensity ratio of 2: 1.With the aid of FAB-MS, as well as the chemical shift values of the H-NMR signals, the antimutagen was identified as 3, 5-dicaffeoylquinic acid.At a dose of 0.5mg/plate, the compound with S9mix inhibited by 66% the mutagenicity of Trp-P-1.
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