Cytological diagnosis of low-grade urothelial carcinoma, with special reference to methods of urine specimen preparation

概要

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Objective: We attempted to devise a suitable method of smear preparation to detect low grade urothelial carcinomas. Study Design: We examined 170 and 50 voided urine specimens with corresponding histological diagnosis of urothelial carcinoma Gradel of the former and false suspicious of the latter. Four methods of preparation of these urine samples were group A: centrifugal direct smear by autosmear; group S: two slides pull method using centrifuge sediment; group SS: group S with spray fixation; group C: centrifugal direct smear by cytospin. In group A, group S and group SS, cytology slides were stained by the Papanicolaou method and May-Giemsa method. In group C, cytology slides were stained by the Papanicolaou method alone. The results of cytological diagnosis according to the method of preparation and the false-suspicion rate were analyzed using the x2 test. Results: No significant differences were found among the four methods, but the false-suspicion rate was higher in group A than in group SS (p<0.05). Conclusion: (1) The two slide-pull method using centrifuged sediment yielded as good results as the centrifugal direct method by cytospin and autosmear.(2) The two slide-pull method is more suitable for Giemsa stain preparation than centrifugal direct method by cytospin and autosmear.
特定非営利活動法人日本臨床細胞学会の論文