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The Auto-Cyto-Screener, an apparatus for the automatic screening of vaginal smears stained by Feulgen reaction has been developted. This machine which employs a color TV camera as a sensor, can discriminate cancer cells from benign squamous epithelial cells, leucocytes and other components by measuring nuclear size and density. The slide specimens to be screened by this apparatus must, therefore, be prepared so that all the cells on the slide glass surface are perfectly dispersed and uniformly distributed. A number of reports have been previously presented on the special method for preparing such specimens. However, the method employed in this study is not perfect yet and improvement are being made for its perfection. This report consists of the work we have recently made on the following two points.<BR>1. Poly-L-lysine coating of the slide glass is extremely effective in preventing cell exfoliation from the slide glass surface and furthermore is useful in maintaining uniform cell distribution on the slide glass surface. It is therefore strongly recommended to incorporate this step in the preparation of vaginal smears for automatic screening.<BR>2. In the preparation of slide specimens using smear suspension from cervical cancer cases, the removal of leucocytes and others by the spontaneous sedimentation technique using single layer Ficoll gradient introduces a slight decrease in total squamous cell count in the smear. However, in most of the cases the ratio of malignant cell count to the total squamous cell count either increase or show almost no difference from that of control specimens. Thus, this procedure seems to be of extremely useful stepin preparing vaginal smears for automatic screening.
- 特定非営利活動法人 日本臨床細胞学会の論文
特定非営利活動法人 日本臨床細胞学会 | 論文
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