Study on the oblective diagnosis by means of exfoliative cytology utilizing fluorospectrophotometry
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概要
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At the present time, the cytological diagnosis isestablished by the subjective recognition of the morphological differences between the normal and themalignant cells utilizing the optical microscope. However, it is quite difficult with this method to managea large number of specimens both quickly and efficiently.<BR>Therefore, the automation of cytological diagnosishas been anxiously sought for by analizing the characteristics of neoplastic cells quantitatively. In orderto materialize the automatic diagnosis, first of all, it is mandatory for us to pick up all the necessarycharacteristics of the malignant cells and arrangethem into really objective criteria.<BR>Fluorospectrophotometry will be able to determinethe degree of fluorescence being adsorbed by thenuclear DNA. Of a few obvious characteristics ofmalignant cells which may be obtained quantitatively, the nuclear density, or the DNA content ofthe neucleus, will be determined by the above technique. Thus, the author actually counted the degree of fluorescence concerning with the nuclearDNA of various cells in the total of 134 smear specimens with AO staining by means of the Nikon'sfluoromicroscope, a modified type of SUR-F at thewave-length of 530 mu.<BR>The followings are the results.<BR>1) The area of determination was 15 square microns and the time required for each single cell wasless than one minute. The degree of fluorescence atthe background and that of a leucocyte were adjusted as 0 and 10 respectively, then the relative valueof fluorescence was calculated by means of the smallsampling theory: The upper limits of rejection were20.37 for normal cells, 37.63 for dyskaryotic cellsand 65.30 for malignant cells.<BR>2) 40 cases of invasive cancer all revealed thedegree of fluorescence over 20.37 and 38 cases ofwhich (i.e. 95%) disclosed the value above 37.63.10 cases of carcinoma in situ all revealed the fluorescence over 20.37 and 7 cases of which (i.e. 70%) disclosed the value above 37.63. 6 cases of displasiaall revealed the fluorescence over 20.37 and 2 casesof which (i.e. 33.3%) were above 37.63.<BR>3) The author classified those cases with the degree of fluorescence less than 20.37 as negative, cases ranging from 20.37 to 37.63 as suspicious andabove 37.63 as positive respectively. Blind test wascarried out on 50 cases applying the above classification in comparison with the degree of ordinaryPapanicolaou smear. 94.5% of negative cases byfluorospectrophotometry accorded with the negativePapanicolaou smear. Likewise, both suspicious andpositive cases by fluorospectrophotometry exactlyaccorded with those by Papanicolaou smear.<BR>4) By establishing the uppper limits of rejectionas to the degree of fluorescence for nuclear DNA ofvarious cells which appear in the smear specimensobtained from the uterine cervix, the fluorospectrophotometry will be able to differentiate the normal, dyskaryotic and malignant cells quite objectively, thus providing a promising future for the automation of cytological diagnosis.
- 特定非営利活動法人 日本臨床細胞学会の論文
特定非営利活動法人 日本臨床細胞学会 | 論文
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