Purification of M. leprae with Special Reference to the Effects of Purified M. leprae Vaccines on Host Macrophage Cell Functions

概要

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M. leprae purified by the method of Draper (IMMLEP Protocol 1/79) based on Percoll gra-dient centrifugation and aqueous two phase separation system and the method of Mori et al. based on the Percoll gradient ultracentrifugation were compared for some of their biological activities. The integrity of purified leprosy bacilli was higher in the latter preparation than the former. When the vaccines prepared from M. leprae cells obtained by the two purifica-tion methods by heat-treatment at 100°C were studied for their efficacy to modulate host macro-phage cell functions, the two vaccines were able to potentiate the phagocytic and active oxy-gen generating abilities but unable to augment the antilistierial activity of the host macropha-ges by double injections 21 days and 4 days before harvest via subcutaneous and intraperitoneal routes, respectively. M. leprae vaccine obtained by Draper's method more markedly height-ened the phagocytic activity of host macrophages than that prepared by Mori's meth d, where-as the inverse was the case for enhancement of the active oxygen producing ability of the host macrophages.
日本ハンセン病学会の論文