夏柑果汁中のアシルアミノ酸の分離について

概要

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Authors have felt a great interest in the plant biological actions with relation to acylamino acids. However acylamino acids as the natural products were extracted from the Natsumikan juice for explanation of a role in the plant. Ionexchange chromatography (Amberlite IRA-400[R2CO-3], Amberlite IRC-50 [H+], Dowex 50-X 8 [H+]) and paper partition chromatography (BuOH : AcOH : water : 4: 1: 5 v/v) used authentic reagents were carried out beforehand, then natural acylamino acids were separated respectively. Quantitative analysis of saponified acylamino acids were carried out by an amino acid autoanalyzer. As the results, acylamino acids that connected with amino groups of amino acids such as glycine, glutamic acid aspartic acid, alanine, serine, proline, lysine, threonine were clearly recognized the existence in the supernatant fraction, whereas the fatty acid portions would identify after this.
公益社団法人日本栄養・食糧学会の論文