Colorimetric Determination of Hydroxyurea with 8-Hydroxyquinoline

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Hydroxyurea is tentatively suggested to be one of the most reasonable reaction product from urea by urea dehydrogenase. Because hydroxyurea has been colorimetrically estimated with p-dimethylamino-benzaldehyde as urea or its derivatives, a procedure for independent estimation of the former from the latters has been required. It was observed that hydroxyurea turns yellowish green with 8-hydroxy-quinoline similar to hydroxylamine, while urea and its derivatives not. In the absorption spectrum of the colored solution of hydroxyurea, a peak is observed at 425 mμ and no absorption over about 600 mμ. On the other hand, in the case of hydroxylamine, the main peak at 710 mμ and the second one at 425 mμ are determined. By discussing some conditions such as buffers, sodium carbonate, coloring temperature and time, the method was established as follows for colorimetry of hydroxyurea, by which urea and its derivatives can not be determined. A mixture of 1 ml hydroxyurea, 1 ml 0.2 M Tris buffer (pH 7.5), 1 ml 1 % 8-hydroxyquinoline, 1 ml 1 M Na2CO3 and H2O to be 5 ml of total volume was incubated at 40°C for 3 hours and the optical density was estimated at 425 mμ. The absorbancy is proportional to concen-tration of hydroxyurea in a range between 5 X 10-5 M and 10-3 M. However, because similar and much intensive coloration was brought about by hydroxylamine, esti-mation of hydroxyurea was disturbed, although the colorimetry of hydroxylamine was not interferred by hydroxyurea. In addition, similar coloration was observed with several substances such as phenyl-hydrazine, semicarbazide and others, which also disturb the estimation of hydroxyurea.
公益社団法人日本栄養・食糧学会の論文

Colorimetric Determination of Hydroxyurea with 8-Hydroxyquinoline

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概要

公益社団法人日本栄養・食糧学会 | 論文

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Colorimetric Determination of Hydroxyurea with 8-Hydroxyquinoline

スポンサーリンク

概要

公益社団法人 日本栄養・食糧学会 | 論文

スポンサーリンク

公益社団法人日本栄養・食糧学会 | 論文