Protein Nutrition and Growth in the Rat
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概要
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It had been found that feeding of albino rats with a diet containing 10, 18 and 27% of protein resulted in marked difference of growth at 10 to 15 weeks after birth. Examinations were made on the difference in the effect of quantity and quality of proteins, that is, the composition of amino acids, on the growth of albino rats fed with 10% or 18% of casein.<BR>336 female rats were divided in 4 groups. Casein was used as the basal protein source at the 10% level. Rats in Group 1 were fed 10% casein and amino acid mixture of "essentials" and "non-essentials" such as occurred in 8% casein as the protein source. Group 2 rats were fed 10% casein only. Group 3 rats were fed 10% casein and isonitrogenous non-essential amino acid mixture corresponding to 8% casein. Group 4 rats were fed the same diet as that for Group 1, but one essential amino acid was removed from the amino acid mixture, maintaining a constant nitrogen level by the substitution of non-essential amino acids. In the case of the deprivation of methionine, cystine was also excluded and in the deprivation of phenylalanine, also tyrosine. Litter mates were distributed to all the four groups. For each diet studied, 4 to 10 rats were employed. The amount of diet intake was made to be same as much as possible but not stricktly pair fed. The experiments were carried out for 16 to 40 weeks.<BR>The difference of growth in terms of body weight and tail length between rats in Group 2 and Group 1 is remarkable at 10 to 15 weeks after birth. Such difference was seen between 10% casein fed group and the other groups (non-essential amino acid group and one essential amino acid-deficient group). On the contrary, there was no significant difference among the Groups 1, 3, and 4.<BR>Tail length and body weight of rats in each group were almost the same at the beginning of the experiment, and the ones that were in some degree larger tended to be larger also at maturity. Tail length and body weight of rats in Group 2 were less than those of rats in other groups on the average at maturity. When this tendency in Group 2 is present at the beginning of the experiment with other groups, the difference becomes marked at maturity. Even if the tail length and body weight of Group 2 rats were larger at the beginning as compared with valine-, isoleucine- and leucine-deficient groups, Group 2 rats were smaller than rats of these groups on the average, at a later stage. A high correlation (r=0.673) existed between tail and shin bone lengths, as observed by using 37 rats. Smear test was done with some rats, for studying the effect of nutrition on the estrus cycle. Estrus cycles of the 10% casein-fed rats appeared later and irregular as compared with other group rats. In addition, some of the rats were sacrificed, and constituents of the body were determined. Body of 10% casein fed rats contained more water and less fat than that of other group rats. As results, difference in growth between 10% and 18% casein-fed rats is due rather to that of the quantity of protein, and not to the quality of protein.
- 公益社団法人 日本栄養・食糧学会の論文
公益社団法人 日本栄養・食糧学会 | 論文
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