Spectrophotometrical Measurement of Instantaneous Calcium Binding of the Relaxing Factor of Muscle

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概要

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• 1. Murexide used in combination with the double-beam spectrophotometer made it possible to directly record the quick changes of Ca ion concentraction in a suspension of relaxing vesicles, viz., isolated sarcoplasmic reticulum. 2. In the presence of Mg ions and ATP, Ca ions were instantaneously bound to the relaxing vesicles. 3. The effects of Ca binding inhibitor, such as anions, lower aliphatic alcohols and detergents, were studied. 4. The mechanism of Ca binding of the relaxing vesicles was discussed.

• 社団法人 日本生化学会の論文

著者

• Ebashi Setsuro

  Department Of Pharmacology Faculty Of Medicine University

• OHNISHI Tsuyoshi

  Department of Nephrology, Shuwa General Hospital

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• Spectrophotometrical Measurement of Instantaneous Calcium Binding of the Relaxing Factor of Muscle

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