Dynamics of Golgi Matrix Proteins after the Blockage of ER to Golgi Transport

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概要

• 論文の詳細を見る

• When the ER to Golgi transport is blocked by a GTP-restricted mutant of Sarlp (H79G) in NRK-52E cells, most Golgi resident proteins are transported back into the ER. In contrast, the cis-Golgi matrix proteins GM 130 and GRASP 65 are retained in punctate cytoplasmic structures, namely Golgi remnants. Significant amounts of the medial-Golgi matrix proteins golgin-45, GRASP55 and giantin are retained in the Golgi remnants, but a fraction of these proteins relocates to the ER. Golgin-97, a candidate trans-Golgi network matrix protein, is retained in Golgi remnant-like structures, but mostly separated from GM 130 and GRASP 65. Interestingly, most Sec13p, a COPII component, congregates into larger cytoplasmic clusters soon after the micro-injection of Sar1p (H79G), and these move to accumulate around the Golgi apparatus. Sec13p clusters remain associated with Golgi remnants after prolonged incubation. Electron microscopic analysis revealed that Golgi remnants are clusters of larger vesicles with smaller vesicles, many of which are coated. GM 130 is mainly associated with larger vesicles and Sec13p with smaller coated vesicles. The Sec13p clusters disperse when p115 binding to the Golgi apparatus is inhibited. These results suggest that cis-Golgi matrix proteins resist retrograde transport flow and stay as true residents in Golgi remnants after the inhibition of ER to Golgi transport.

• 社団法人 日本生化学会の論文

著者

• Mihara Katsuyoshi

  Department Of Molecular Biology Graduate School Of Medical Science

• SOHDA Miwa

  Department of Biochemistry, Fukuoka University School of Medicine

• Misumi Yoshio

  Department Of Biochemistry Fukuoka University School Of Medicine

• Yoshimura Shin-ichiro

  Molecular Biology Laboratory Faculty Of Pharmaceutical Sciences Kanazawa University

• Barr Francis

  Department Of Cell Biology Max-planck-institute Of Biochemistry

• Fujii Gourou

  Cancer Research Institute Kanazawa University

• Shakoori Abbas

  Cancer Research Institute Kanazawa University

• Nakamura Nobuhiro

  Molecular Biology Laboratory Faculty Of Pharmaceutical Sciences Kanazawa University

• Ohno Hiroshi

  Cancer Research Institute Kanazawa University

• Yamamoto Akitsugu

  Department Of Animal Bioscience Faculty Of Bio-science Nagahama Institute Of Bioscience And Technology

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