A Temperature-Sensitive Mutant of the Mammalian RNA Helicase, DEAD-BOX X Isoform, DBX, Defective in the Transition from G 1 to S Phase
スポンサーリンク
概要
- 論文の詳細を見る
ts ET 24 cells are a novel temperature-sensitive (ts) mutant for cell proliferation of hamster BHK 21 cells. The human genomic DNA which rescued the temperature-sensitive lethality of is ET 24 cells was isolated and screened for an open reading frame in the deposited human genomic library. X chromosomal DBX gene encoding the RNA helicase, DEAD-BOX X isoform, which is homologous to yeast Ded1p, was found to be defective in this mutant. The single point mutation (P267S) was localized between the Motifs I and la of the hamster DBX of is ET 24 cells. At the nonpermissive temperature of 39.5°C, is ET 24 cells were arrested in the G 1-phase and survived for more than 3 days. In is ET 24 cells, total protein synthesis was not reduced at 39.5°C for 24 h, while mRNA accumulated in the nucleus after incubation at 39.5°C for 17 h. The amount of cyclin A mRNA decreased in is ET 24 cells within 4 h after the temperature shift to 39.5°C, consistent with the fact that the entry into the S-phase was delayed by the temperature shift.
- 社団法人 日本生化学会の論文
著者
-
Nishimoto Takeharu
Department Of Molecular Biology Graduate School Of Medical Science Kyushu University
-
Sekiguchi Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
-
Noguchi Eishi
Department Of Molecular Biology Graduate School Of Medical Science Kyushu University
-
Fukumura Junko
Department Of Molecular Biology Graduate School Of Medical Science Kyushu University
関連論文
- Preparation of New Nitrogen-Bridged Heterocycles 67. Syntheses of α,α′-Bis[(thieno[3,4-b]indolizin-3-yl)thio]-o-, m-, and p-xylene Derivatives and Their Conformational Structures
- Crystal Structure of Eucaryotic E3, Lipoamide Dehydrogenase from Yeast^1
- Crystallization of Eukaryotic E3, Lipoamide Dehydrogenase, from Yeast, for Exhibiting X-Ray Diffraction beyond 2.5 A Resolution, and Preliminary Structure Analysis
- Conserved Histidine Residues of RCC1 Are Essential for Nucleotide Exchange on Ran
- Crystal Structure of 3-Isopropylmalate Dehydrogenase from the Moderate Facultative Thermophile, Bacillus coagulans: Two Strategies for Thermostabilization of Protein Structures
- RCC1 in the Ran Pathway
- An Altered Nuclear Migration into the Daughter Bud is Induced by the Cyclin A1-mediated Cdc28 Kinase through an Aberrant Spindle Movement in Saccharomyces cerevisiae
- A Subunit of the Mammalian Oligosaccharyltransferase, DAD1, Interacts with Mcl-1, One of the bcl-2 Protein Family^1
- L^-theory of noncausal stochastic integrals
- Regulation of nucleocytoplasmic transport and cell proliferation by Ran GTPase
- A Hamster Temperature-Sensitive Alanyl-tRNA Synthetase Mutant Causes Degradation of Cell-Cycle Related Proteins and Apoptosis
- A Temperature-Sensitive Mutant of the Mammalian RNA Helicase, DEAD-BOXX Isoform, DBX, Defective in the Transition from G1 to S Phase
- Human Dis3p, Which Binds to Either GTP-or GDP-Ran, Complements Saccharomyces cerevisiae dis3^1
- Disruption of the YRB2 Gene Retards Nuclear Protein Export, Causing a Profound Mitotic Delay, and Can Be Rescued by Overexpression of XPO1/CRM1
- Inhibition by Anti-RCC1 Monoclonal Antibodies of RCC1-Stimulated Guanine Nucleotide Exchange on Ran GTPase
- A remark on L^∞ in the space of BMO-martingales
- SYNTHESIS OF 3-O-α-D-GLCOPYRANOSYL-D-GLUCOSE (SAKEBIOSE OR NIGEROSE) I. REACTION OF 1, 2-5, 6-DI-O-ISOPROPYLIDENE-D-GLUCOFURANOSE AND 2, 3, 4, 6-TETRA-O-ACETYL-α-D-GLUCOPYRANOSYL BROMIDE
- Remarks on a criterion for the uniform integrability of positive local martingales
- Crystal Structure of Eucaryotic E3, Lipoamide Dehydrogenase from Yeast.
- A Temperature-Sensitive Mutant of the Mammalian RNA Helicase, DEAD-BOX X Isoform, DBX, Defective in the Transition from G 1 to S Phase