The Rate of Cell Growth Is Regulated by Purine Biosynthesis via ATP Production and G1 to S Phase Taansition.

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概要

• 論文の詳細を見る

• We recently showed that an increased supply of purine nucleotides increased the growth rate of cultured fibroblasts. To understand the mechanism of the growth rate regulation, CHO Kl (a wild type of Chinese hamster ovary fibroblast cell line) and CHO ade -A (a cell line deficient in amidophosphoribosyltransferase, a rate-limiting enzyme of the de novo pathway) were cultured under various conditions. Moreover, a defective de novo pathway in CHO ade -A cells was exogenously restored by 5-amino-4-imidazole-carboxamide riboside, a precursor of the de novo pathway. The following parameters were determined: the growth rate of CHO fibroblasts, the metabolic rate of the de novo pathway, the enzyme activities of amidophosphoribosyltransferase and hypoxanthine phosphoribosyltransferase, the content of intracellular nucleotides, and the duration of each cell-cycle phase. We concluded the following: (i) Purine de novo synthesis, rather than purine salvage synthesis or pyrimidine synthesis, limits the growth rate. (ii) Purine nucleotides are synthesized preferentially by the salvage pathway as long as hypoxanthine is available for energy conservation. (iii) The GTP content depends on the intracellular ATP content. (iv) Biosynthesis of purine nucleotides increases the growth rate mainly through ATP production and promotion of the G1/S transition.

• 社団法人 日本生化学会の論文

著者

• YOSHIMOTO KATSUHIKO

  Otsuka Department of Clinical and Molecular Nutrition School of Medicine, The University of Tokushim

• MORITANI MAKI

  Otsuka Department of Clinical and Molecular Nutrition School of Medicine, The University of Tokushim

• Miwa Yoshihiro

  Division Of Internal Medicine Public Nanokaichi Hospital

• Kondo Maki

  Otsuka Department Of Molecular Nutrition School Of Medicine The University Of Tokushima

• HONDA Soichi

  Otsuka Department of Molecular Nutrition, School of Medicine,The University of Tokushima

• Katashima Rumi

  Otsuka Department Of Molecular Nutrition School Of Medicine The University Of Tokushima

• Yamaoka Takashi

  Dentistry Institute for Genome Research. The University of Tokushima

• Hayashi Yoshio

  Division of Genetic Information, Institute for Genome Research. The University of Tokushima

• Itakura Mitsuo

  Dentistry Institute for Genome Research. The University of Tokushima

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