Induction of Apoptosis by Bufalin in Human Tumor Cells Is Associated with a Change of Intracellular Concentration of Na+ Ions.

スポンサーリンク

概要

• 論文の詳細を見る

• In an attempt to characterize the mechanisms that are operative at the early stages of the induction of apoptosis by bufalin, a component of the traditional Chinese medicine chan'su, we examined the effects of bufalin on plasma membrane potential, as determined by monitoring the uptake by cells of rhodamine 123. Bufalin induced apoptosis in human monocytic leukemia THP-1 cells, in human lymphoblastic leukemia MOLT-3 cells, and in human colon adenocarcinoma COLO320DM cells but not in normal human leukocytes, for example, polymorphonuclear cells and lymphocytes, and not in murine leukemia P388D1 and M1 cells. Treatment for 3h with bufalin at 10-6M caused a decrease in the plasma membrane potential in several lines of human tumor cells but not in murine leukemia cells. No changes in mitochondrial membrane potential, as monitored with the fluorescent dye JC-1, and no release of cytochrome c were observed within at least 6h after the start of treatment with bufalin. Moreover, overexpression of bel-2 in human leukemia HL60 cells that had been transfected with cDNA for bcl-2 prevented bufalin-induced apoptosis but had no significant effect on the change in plasma membrane potential induced by bufalin. Since bufalin specifically inhibits the Na+, K+-ATPase of human but not murine tumor cells, and since this inhibition leads to a change in intracellular concentration of Na+ ions, our findings suggest that bufalin induces apoptosis in human tumor cells selectively via inhibition of the Na+, K+-ATPase, which acts upstream of the bcl-2 protein.

• 社団法人 日本生化学会の論文

著者

• NAKAJO Shigeo

  Laboratory of Biochemistry, Yokohama College of Pharmacy

• Masuda Yutaka

  Laboratory Of Biological Chemistry School Of Pharmaceutical Sciences Showa University

• Nakaya Kazuyasu

  Laboratory Of Biological Chemistry School Of Pharmaceutical Science Showa University

• KAWAZOE Nobuko

  Laboratory of Biological Chemistry

• Aiuchi Toshihiro

  Analysis Center, School of Pharmaceutical Science, Showa University

関連論文

• Mechanism of inhibition of tumor angiogenesis by β-hydroxyisovalerylshikonin
• β-Hydroxyisovalerylshikonin Inhibits the Cell Growth of Various Cancer Cell Lines and Induces Apoptosis in Leukemia HL-60 Cells through a Mechanism Defferent from Those of Fas and Etoposide
• Localization and gene expression of the receptor for pituitary adenylate cyclase-activating polypeptide in the rat brain
• HS-142-1,a Novel Non-peptide Antagonist for Atrial Natriuretic Peptide Receptor, Selectively Inhibits Particulate Guanylyl Cyclase and Lowers Cyclic GMP in LLC-PK_1 Cells
• Immunocytochemical localization of nicotinic acetylcholine receptor in the rat cerebellar cortex
• β-Hydroxyisovalerylshikonin Is a Novel and Potent Inhibitor of Protein Tyrosine Kinases
• Differential Binding Activity of Erythrocyte Ankyrin to the Alpha-Subunits of Na^+, K^+-ATPases from Rat Cerebral and Axonal Membrane
• Reducing Activity of Colloidal Platinum Nanoparticles for Hydrogen Peroxide, 2,2-Diphenyl-1-picrylhydrazyl Radical and 2,6-Dichlorophenol Indophenol (Medicinal Chemistry)
• Comparative Studies on the Primary Structure of Human Cystatin As from Epidermis, Liver, Spleen, and Leukocytes
• Geranylgeraniol Is a Potent Inducer of Apoptosis in Tumor Cells
• Increase in Intracellular Ca^ Concentrations and the Corresponding Intracellular Acidification Are Early Steps for Induction of Apoptosis by Geranylgeraniol in HL60 Cells(Molecular and Cell Biology)
• Induction of Apoptosis by Bufalin in Human Tumor Cells Is Associated with a Change of Intracellular Concentration of Na^+ Ions
• Zine Ions Prevent Processing of Caspase-3 during Apoptosis Induced by Geranylgeraniol in HL-60 Cells
• Uptake of 1-Methyl-4-phenylpyridinium Ion (MPP^+) and ATP Content in Synaptosomes
• DISTRIBUTION OF 1-METHYL-4-PHENYLPYRIDINE, WHICH INHIBITS RESPIRATORY CHAIN, IN MITOCHONDRIA
• Induction of Apoptosis by Bufalin in Human Tumor Cells Is Associated with a Change of Intracellular Concentration of Na+ Ions.
• Zinc Ions Prevent Processing of Caspase-3 during Apoptosis Induced by Geranylgeraniol in HL-60 Cells.

もっと見る 閉じる

スポンサーリンク