Reduced Induction of HSP70 in PC12 Cells during Neuronal Differentiation.
スポンサーリンク
概要
- 論文の詳細を見る
Rat pheochromocytoma PC12 cells differentiate into nonreplicating neuronal cells with neurite extensions in response to nerve growth factor (NGF). To gain better understanding of the regulation of stress responses in neuronal cells, we examined the induction of HSP70, HSP70 mRNA, and heat shock factor 1(HSF1) DNA-binding activity following treatment by heat shock or with sodium arsenite or amino acid analogue in PC12 cells treated with or without NGF. The induction of HSP70 and HSP70 mRNA following these stresses was diminished in the differentiated PC12 cells compared to the undifferentiated cells, whereas the HSF1 DNA-binding activity was enhanced in the differentiated PC12 cells. This phenomenon was characteristic of the differentiated neuronal cells rather than growtharrested cells. Thus, neuronal cells appear to show an altered stress response depending on their differentiation state, and the diminished HSP70 expression in the differentiated neuronal cells may explain the sensitivity of neuronal cells to pathophysiological stressors.
- 社団法人 日本生化学会の論文
著者
-
Takahashi Hisaaki
Department Of Biochemistry Kyoto Pharmaceutical University
-
Yamagishi Nobuyuki
Department of Biochemistry & Molecular Biology, Division of Biological Sciences, Kyoto Pharmaceutical University
-
Hatayama Takumi
Department of Biochemistry & Molecular Biology, Division of Biological Sciences, Kyoto Pharmaceutical University
関連論文
- Hsp105α Enhances Stress-Induced Apoptosis but Not Necrosis in Mouse Embryonal F9 cells
- Characteristic Expression of High Molecular Mass Heat Shock Protein HSP105 during Mouse Embryo Development
- Synthetic small interfering RNA targeting heat shock protein 105 induces apoptosis of various cancer cells both in vitro and in vivo
- DNA vaccination of HSP105 leads to tumor rejection of colorectal cancer and melanoma in mice through activation of both CD4^+ T cells and CD8^+ T cells
- Increased Radiosensitivity of p16 Gene-deleted Human Glioma Cells after Transfection with Wild-type p16 Gene
- Mutation Induction by Extremely Low Frequency Magnetic Fields
- Changes in Radiation Sensitivity of Human Osteosarcoma Cells after p53 Introduction
- Enhanccd expression of 13-galactosidase gene by cxtrcmely low frequency magnetic field in PCVG cells.
- Reduced Induction of HSP70 in PC12 Cells during Neuronal Differentiation
- Nuclear Localization Mechanism of Hsp105β and its Possible Function in Mammalian Cells
- Effects of Hyperosmotic NaCl and Glycerol Stress on Stress Response of Human HeLa Cells
- Characteristic Experssion of 105-kDa Heat Shock Protein (HSP105) in Various Tissues of Nonstressed and Heat-Stressed Rats
- Production of Phytochelatins in Polygonum cuspidatum on Exposure to Copper but not to Zinc
- Purification and Some Properties of Copper Reductase from Cell Surface of Debaryomyces hansenii
- Solubilization and Properties of Copper Reducing Enzyme Systems from the Yeast Cell Surface in Debaryomyces hansenii
- Naringenin Inhibits the Aggregation of Expanded Polyglutamine Tract-Containing Protein through the Induction of Endoplasmic Reticulum Chaperone GRP78
- New methods for synthesis of .ALPHA.,.BETA.-unsaturated carboxylic esters from carbonyl compounds using monoanions of dithiocarbonates, and dianions of ethyl mercaptoacetate and ethyl 2-mercaptopropionate.
- Reduced Induction of HSP70 in PC12 Cells during Neuronal Differentiation.