Nucleotide Sequence of the Promoter Region of Chicken Cytosolic Phosphoenolpyruvate Carboxykinase Gene.

概要

論文の詳細を見る
The nucleotide sequence of the promoter region and its flanking regions which span -1855to +2083 in the chicken cytosolic phosphoenolpyruvate carboxykinase gene was determined. The transcription initiation site was located at 119 nucleotides downstream of thepreviously reported chicken kidney transcription initiation site of this gene. The nu-cleotide sequences of exons 1, 2, and 3 were highly homologous to the corresponding exonsof the rat gene. Homology of the sequence -1 to -500 to that of rat gene was 52% and mostof the hormone-responsive sequences in rat gene, such as the glucocorticoid-responsiveregion, were not conserved in the chicken gene, in accord with the species-specificresponsiveness to starvation. In contrast, in the region of -1 to -300, some sequence motifsconserved both in the chicken and rat genes were found at essentially the same positions inthe promoters. Such sequence motifs included a cAMP-responsive element (CRE), a nuclearfactor-1 (NF-1/CTF)-binding site, and a hepatocyte nuclear factor-1 (HNF-1)-binding site. Transient expression of the reporter luciferase gene ligated to the 3' end of this chickensequence ( -1855 to +7) was observed in a primary culture of chick hepatocytes whendibutyryl cyclic AMP was added to the culture medium.
社団法人日本生化学会の論文