Isolation and Characterization of the Human Chromosomal Gene for Prostacyclin-Stimulating Factor.
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概要
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Prostacyclin-stimulating factor (PSF) is a protein which acts on vascular endothelial cells and stimulates the production of prostacyclin. Recently, we were able to purify PSF from the conditioned medium of cultured human diploid fibroblasts and clone PSF cDNA. In this study, we screened a human genomic library and isolated genomic clones to determine the structure of the human chromosomal PSF gene. By determining the nucleotide sequence and transcription initiation site of this gene, we found that it comprises 5 exons and 4 introns. Southern hybridization analysis indicated the presence of a single copy of the PSF gene per haploid set of chromosomes. The 300 by upstream of the transcription initiation site had a very high GC content, and 7 binding sites for the transcription regulating factor Sp 1 were present.
- 社団法人 日本生化学会の論文
著者
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UMEDA FUMIO
Third Department of Internal Medicine Faculty of Medicine Kyushu University
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HASHIMOTO TOSHIHIKO
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University
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SATO Hironori
Biosciences Research Laboratory, Mochida Pharmaceutical Co., Led.
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KANAMORI Toshinori
Biosciences Research Laboratory, Mochida Pharmaceutical Co., Led.
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Nawata Hajime
Third Department Of Internal Medicine Faculty Of Medicine Kyushu Univeristy
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Nii Atsushi
Biosciences Research Laboratory Mochida Pharmaceutical Co. Led.
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Negishi Takaaki
Biosciences Research Laboratory Mochida Pharmaceutical Co. Led.
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Koushima Hiroko
Biosciences Research Laboratory Mochida Pharmaceutical Co. Led.
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Mizushima Seiichi
Biosciences Research Laboratory Mochida Pharmaceutical Co. Led.
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Okamoto Atsushi
Biosciences Research Laboratory Mochida Pharmaceutical Co. Led.
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Nii Atsushi
Biosciences Research Laboratory, Mochida Pharmaceutical Co., Ltd.
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