Development of a High-Performance Liquid Chromatographic Gel Carrying Intercalator-Like Benzoates for Analysis of Oligonucleotides.

概要

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Intercalator-like aromatics-loaded columns were prepared by modifying silica with benzoic acids m-substituted with the nitro (NBA column), hydrogen (BA), methyl (MBA), methoxy (MOBA), and dimethylamino (DABA) groups. These chromatographic supports constitute a hydrophobic stationary phase; they were used for the separation of mono-, di-, and tetranucleotides under isocratic conditions in HPLC. The retention of nucleotides on these column correlated with the π-electron density of the aromatic moiety of these supports, suggesting the importance of stacking interactions of the aromatic rings with the nucleic base. The base-selective separation of nucleoties, CCGG and GGCC, was achieved with the MBA column.
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