(2-Phthalimidyl)benzoyl Azides as Fluorescence Labeling Reagents for Alcohols in High Performance Liquid Chromatography.
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概要
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Two fluorescence labeling reagents, 3- and 4-(2-phthalimidyl)benzoyl azide, were designed for primary and secondary alcohols for high performance liquid chromatography. These reagents, prepared from orthophthalaldehyde and aminobenzoic acid in several steps, react with alcohols at 125°C for 30min to produce the fluorescent derivatives, which can be separated on a reversed-phase column ERC-ODS-1161 with acetonitrile/water (70:30) or acetonitrile/ethanol (70:30) as eluent. The extent of conversion of cholesterol into fluorescent derivative was 100%. The detection limit for the alcohols labeled with 3-(2-phthalimidyl)benzoyl azide was 0.1-0.4pmol per injection (10μl). 3-(2-Phthalimidyl)-benzoyl azide was applied for the determination of cholesterol in human serum by high performance liquid chromatography with fluorescence detection. The values of cholesterol in normal human sera were 4.8-9.4μmol/ml.
- 社団法人 日本分析化学会の論文
社団法人 日本分析化学会 | 論文
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