Micelle-enhanced lucigenin chemiluminescence for the determination of catecholamines.

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Aqueous solutions of cationic micellar hexadecyltrimethylammonium hydroxide (CTAOH) and nonionic micellar polyoxyethylene(23) dodecanol enhanced the intensity of maximum chemiluminescence (CL) light emission from the reaction of 10, 10′-dimethyl-9, 9′-biacridinium dinitrate (lucigenin) with catecholamines. In contrast, a lowering of the CL emission was observed in the presence of anionic micellar sodium dodecylsulfate. The best sensitivity was achieved in micellar CTAOH: logarithmic calibration curves of adrenaline, dopamine and noradrenaline were linear over the range from the detection limit of 1×10-8, 3×10-7 and 5×10-7M up to 1×10-4M, respectively. In the presence of micellar CTAOH, improvements in the sensitivity by factors of 1.6-10 were observed relative to that in water. The relative standard deviation was 2.5% in the micellar media.
社団法人日本分析化学会の論文