Fluorometric determination of xanthurenic acid in urine with calcium nitrate and diethylamine.
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The fluorescence of xanthurenic acid was found to be greatly enhanced after heating in the methanol solution of 25%(v/v) diethylamine and 3mM calcium nitrate. This finding was successfully applied to the assay procedure of xanthurenic acid in urine. The fluorescence intensity of xanthurenic acid by the calcium nitrate-diethylamine method was measured at 485nm with excitation at 375nm. The calibration curve for xanthurenic acid showed a linear relation in the range of 50pmol/ml to 1.5nmol/ml. The relative standard deviation at 1.5nmol was 2.9% (n=6). In this procedure, recovery of xanthurenic acid in the five samples of urine was found to be 102.0±6.2% (n=5). Xanthurenic acid contents in urine of healthy adults which were collected for 24h were 12.4±2.1μmol/d (male, 39 years old, n=5) and 7.5±1.9μmol/d(female, 31 years old, n=5).
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社団法人 日本分析化学会 | 論文
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