Chemiluminescent assay of hydrogen peroxide using leuco-2',7'-dichlorofluorescein-peroxyoxalate.
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概要
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A new chemiluminescence system for the determination of hydrogen peroxide (H2O2) was developed. The assay principle of this system is that fluorescent chromophore produced from leuco-2′, 7′-dichlorofluorescein (LDCF) by the reaction with H2O2 and peroxidase can be made luminescent by addition of bis(2, 4, 6-trichlorophenyl) oxalate (TCPO) and H2O2 dissolved in acetonitrile. So, H2O2 could be determined at a concentration of 10-9 to 10-5M as the amount of fluorescent chromophore, dichlorofluorescein (DCF). To show one application of this system, peroxidase activity was determined in the range of 10-3 to 1U/ml. Uric acid in presence of uricase was also determined in the range of 5×10-5 to 5×10-2mg/dl.
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