Glutamate Sensors Carrying Glutamate Oxidase/Peroxidase Bienzyme System on Tin Oxide Electrode.
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概要
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Amperometric L-glutamic acid sensors were fabricated by depositing L-glutamate oxidase (GLOD) and horseradish peroxidase (HRP) on the tin oxide surface by means of either encapsulation into electropolymerized pyrrole or sequential chemical modification via surface hydroxyl groups. After optimization for the mixing ratio of the two enzymes during polypyrrole (PPy) formation and for the thickness of the PPy film, the GLOD/HRP/PPy electrode gave a nearly linear response to L-glutamic acid in the concentration range of 10-7 to 10-4M, with a response time of about 1min. The GLOD/HRP bilayer-modified electrode showed a sensitivity comparable to that of the PPy-encapsulated bienzyme electrode. Both type of electrodes exhibited excellent substrate specificity. The pH dependence and lifetime of the sensor response are also given.
- 社団法人 日本分析化学会の論文
社団法人 日本分析化学会 | 論文
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