The Kinetic Study of Labile Protein in the Adult Rats
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概要
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This experiment dealt with a kinetic analysis on the metabolism of labile protein with respect to the pool size of protein metabolism in adult rats. Two kinds of 20% and 5% protein diets were prepared with each of the purified whole egg protein and the <SUP>15</SUP>N-Chlorella protein supplemented with L-methionine which served as a tracer at the termination of experiments. The 20% protein diet (20% group), the 5% protein diet following the 20% protein diet (5% group) and the 20% protein diet following the 5% protein diet (R 20% group, i. e. under repletion) were given to the animals. The results obtained from the 15N measurement were as follows:<BR>1) The size of nitrogen metabolic pool (mgN) was 286 in the 20% group, 249 in the 5% group and 304 in the R 20% group. These values reflected the amounts of labile protein in the three groups.<BR>2) The size of more active protein pool (mgN) was 208 in the 20% group, 119 in the 5% group and 222 in the R 20% group, where the decrease in the 5% group was remarkable, also corresponding to the decrease in labile protein of this group.<BR>3) The size of less active protein pool (mgN) was 9, 506 in the 20% group, 9, 579 in the 5% group and 9, 669 in the R 20% group. Thus, a difference was not observed between the groups.<BR>4) From these results, it was clarified that the larger part of more active protein pool and some part of nitrogen metabolic pool constitute labile protein in the body.<BR>5) 15N was highly recovered in pancreas and small intestine in the 20% and R 20% groups, whereas in hair and skin in the 5% group, showing a metabolic flow of labile protein in the body.
- 公益社団法人 日本栄養・食糧学会の論文
公益社団法人 日本栄養・食糧学会 | 論文
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