ビタミンB1欠乏時におけるラット肝細胞のRNAならびにタンパク質合成について
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概要
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Rats fed on thiamine-deficient diet showed a slight increase in body weight for the first two weeks but the weight began to decrease thereafter and the weight was lower than the initial weight after 31 days. The body weight recovered by the oral administration of thiamine.<BR>Wet weight of the liver from rats fed on thiamine-deficient diet showed a slight increase for the first 18 days but not thereafter.<BR>Majority of thiamine in liver cells was present in the cytoplasm and showed a rapid decrease with continuation of thiamine-deficient diet.<BR>Ribosomal pattern of the post-mitochondrial supernatant fraction showed decrease in heavy polysomes and increase in light polysomes after 18 days on thiamine-deficient diet, and this tendency appeared more markedly after 25 to 31 days of thiamine deficiency, but recovered rapidly by the administration of thiamine.<BR>In the purified ribosomal pattern, heavy polysomes tended to increase after 7 days of thiamine deficiency and incorporation of [<SUP>3</SUP>H] orotic acid into the polysomal RNA increased in the polymer portion above trimers. After 25 to 31 days of thiamine deficiency, heavy polysomes decreased and light polysomes increased markedly, and incorporation of [<SUP>3</SUP>H] orotic acid also decreased markedly. Ratio of polysomes of aver hexamers to total polysome showed transitory increase after 7 days of thiamine deficiency but decreased thereafter.<BR>Specific activity of the incorporation of [<SUP>3</SUP>H] orotic acid into the polysomal RNA in vivo and that of the incorporation of [<SUP>14</SUP>C]-leucine into the polysomal RNA <I>in vitro</I> showed a transitory increase after7 days of thiamine deficiency and then decreased day by day. Specific activity af the incorporation of [<SUP>3</SUP>H] orotic acid into nuclear RNA <I>in vivo</I> increased slightly over that of the control after 7 days of thiamine deficiency but there was no great difference and the activity decreased after 18 days of deficiency. Both recovered rapidly by the administration of thiamine.
- 公益社団法人 日本栄養・食糧学会の論文
公益社団法人 日本栄養・食糧学会 | 論文
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