The Effect of the Fortification of Lysine, Threonine, Methionine, and Tryptophan to the Mixture of Rice and Soy Bean Protein

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Forty albino rats were divided into five groups, and fed with the mixture of the polished rice powder, rice protein extracted from the rice powder and raw soy bean powder as a protein source of the basal diet. The ratio of rice and soy bean protein in the diet was 1.6: 1, and the total protein content was ten per cent. The content of four essential amino acids, lysine, threonine, methionine and tryptophan was calculated and compared with the human reference protein. Group A was a control group. The diet of group C was fortified with lysine and threonine to make their content about twice as much as that of the reference protein. The diet of group B was fortified only with lysine. The diet of group D was further fortified with methionine, and that of group E was fortified with the four amino acids. The latter two amino acids were fortified to make the total content twenty per cent as much as that of the reference protein. The rats were fed with these diets for four weeks and the activities of the xanthine oxidase of the liver homogenate were estimated. The averaged activity of the control group (A) was 157, group B 127, group C 121, group D 267 and group E 307, uptaken O2in μl per hour per gram of the liver, respectively. The differences between group E and each of group A, B and C were statistically significant.
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