Changes in Interleukin-1 Production of Peritoneal Macrophages during Estrous Cycle in Golden Hamsters.
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The present study demonstrated the change in interleukin-1 (IL-1) production of peritoneal macrophages during the estrous cycle in golden hamsters and discussed its possible roles in ovarian function. Macrophages were collected from the peritoneal cavity at 0900h on various days of the estrous cycle and incubated for 6h in the presence of ovine pituitary LH (500ng/ml). The IL-1 concentration in the media was measured by bioassay with the A375S2 human melanoma cell line. The number of macrophages significantly (P<0.01) increased on estrus and proestrus compared with diestrus 1 or diestrus 2. LH-induced production of IL-1 was also greater (P<0.01) on proestrus (292±36pg/106cells/ml) and estrus (222±30pg/106cells/ml) than on diestrus 1 (34±15pg/106cells/ml) or diestrus 2 (117± 16pg/106cells/ml). To clarify the factor inducing the changes in peritoneal macrophages, hamsters were ovariectomized on diestrus 1, and 3 weeks later the animals were treated with sc injections of progesterone (200μg/day), testosterone (100μg/day), estradiol (10μg/day) or sesame oil for three days. The hamsters were killed 24h after the last injection, and the number and IL-1 producing capacity of macrophages were determined. The number of macrophages and their response to LH to produce IL-1 were increased significantly (P<0.01) by estradiol treatment but not by progesterone or testosterone treatment. It was concluded that the peritoneal macrophages became more sensitive to LH to produce IL-1 on proestrus and estrus in cyclic hamsters, and that these changes in macrophages, probably induced by estradiol, would play important roles in ovarian function.
- 一般社団法人 日本内分泌学会の論文
一般社団法人 日本内分泌学会 | 論文
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