Syk and Lyn Are Involved in Radiation-Induced Signaling, but Inactivation of Syk or Lyn Alone Is Not Sufficient to Prevent Radiation-Induced Apoptosis.

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概要

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• Radiation-induced biochemical events that mediate the intracellular signal transduction leading to cell apoptosis are largely unknown. Limited evidence suggests the possible involvement of one or more protein-tyrosine kinases (PTKs) in radiation-induced cellular responses, including apoptosis. However, so far, a PTK(s) responsible for the radiationinduced tyrosine phosphorylation of cellular substrates has not been identified and the role of the PTK(s) in the radiation-induced apoptosis remains unclear. To examine the roles of Syk and Lyn in radiation-induced signal transduction and radiation-induced apoptosis, we analyzed Syk-deficient or Lyn-deficient DT40 B cells along with wild-type cells following radiation. When DT40 B cells were exposed to radiation, the activity of Syk kinase dramatically increased and reached a maximum with 0.25 Grays (Gy) (15s), and then decreased, whereas Lyn kinase activity increased and reached a maximum with a dose of 1.00 Gy (1min). However, an apparent difference was not observed in radiation-induced apoptosis among wild-type, Syk-deficient, and Lyn-deficient DT40 B cells. These results indicate that Syk and Lyn kinases are involved in radiation-induced signal transduction, with different kinetics. In addition, our results revealed that functional inactivation of Syk or Lyn alone is not sufficient to prevent radiation-induced apoptosis. Thus, it is suggested that the activation of Syk or Lyn kinase alone may be sufficient to mediate the radiationinduced apoptosis in DT40 B cells, or both kinases may not be required for this biological process.

• 社団法人 日本生化学会の論文

著者

• Wang Xiaoying

  Department Of Biochemistry Kobe University School Of Medicine

• Takata Minoru

  Department Of Dermatology Kanazawa University School Of Medicine

• YANG Cheng

  Department of Biochemistry, Fukui Medical School

• Yanagi Shigeru

  Department Of Electrical & Electronics Engineering National Defense Academy

• Yamamura Hirohei

  Department Of Biochemistry Kobe University School Of Medicine

• Kurosaki Tomohiro

  Department Of Biochemistry Cancer Institute Okayama University Medical School

• MARUYAMA Shingo

  Departhents of Biochemistry and Surgery, Fukui Medical School

• Maruyama Shingo

  Department of Biochemistry, Fukui Medical School, Matsuoka

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