微細針穿孔法による起床時コルチゾール反応の経皮的な評価

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Transdermal assessment of cortisol awakening response (CAR) by microneedle perforation technique was regarded. CAR is a prominent cortisol variation after awakening. Since it has been reported to associate with chronic or daily stress, CAR is assumed as a possible stress indication. However CAR determination by saliva has methodological disadvantages such as lower compliance of multiple specimen collection, bold estimation of total cortisol by discrete sampling time points, etc. We then introduced our developed transdermal interstitial fluid extraction technique, which was implemented by microneedle perforation technique, and investigated the validity of CAR determined by extracted interstitial fluid. Subjects, 10 healthy male, were instructed to collect their saliva at four time points after awakening at 6:00-7:00 and in the afternoon at 14:30-15:30. Besides, their interstitial fluid was collected by microneedle perforation technique on the same schedule. There found a significant positive correlation (<I>r</I> = 0.80, <I>p</I> <.01) between salivary CAR and that of interstitial fluid when it was adjusted by sodium ion concentration which was assumed as a calibrator of the extracted interstitial fluid. The proposed transdermal assessment of interstitial cortisol can be a useful methodology for CAR determination as the minimally invasive manner without any physical pain or bleeding.
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