Deciphering the Roles of Glycan Processing in Glycoprotein Quality Control through Organic Synthesis

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概要

• 論文の詳細を見る

• Protein quality control (QC) in the endoplasmic reticulum (ER) comprises many aspects, including folding and transport of nascent proteins and degradation of misfolded proteins. Recent studies have revealed that high-mannose-type glycans play pivotal roles in the QC process. To gain knowledge of the molecular basis of this process with well-defined homogeneous compounds, we achieved a convergent synthesis of high-mannose-type glycans and their functionalized derivatives. A major part of our study focused on analyses of UDP-Glc: glycoprotein glucosyltransferase (UGGT) and ER glucosidase II, which play crucial roles in glycoprotein QC, to clarify their specificities. In addition, we established an in vitro assay system mimicking the in vivo condition, which is highly crowded due to the presence of various macromolecules.

• 公益社団法人 日本農芸化学会の論文

著者

• Ito Yukishige

  Synthetic Cellular Chemistry Laboratory Riken Advanced Science Institute

• TAKEDA Yoichi

  ERATO Glycotrilogy Project, Japan Science and Technology Agency (JST)

• ITO Yukishige

  Synthetic Cellular Chemistry Laboratory, RIKEN

関連論文

• Demonstration of the pH Sensitive Binding of Multivalent Carbohydrate Ligands to Immobilized Shiga-Like Toxin 1 B Subunits
• Analysis of glycoprotein processing in the endoplasmic reticulum using synthetic oligosaccharides
• Carbohydrate-Binding Molecules with Non-Peptidic Skeletons
• 非ペプチド性の糖結合分子
• Deciphering the Roles of Glycan Processing in Glycoprotein Quality Control through Organic Synthesis

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