Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts
スポンサーリンク
概要
- 論文の詳細を見る
We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase gene harboring tandem copies of an interesting codon at the 5' end.
- 公益社団法人 日本農芸化学会の論文
著者
-
Okumoto Yutaka
Graduate School of Agriculture, Kyoto University
-
Tanisaka Takatoshi
Graduate School of Agriculture, Kyoto University
-
TSUKIYAMA Takuji
Graduate School of Agriculture, Kyoto University
-
Teraishi Masayoshi
Graduate School Of Agriculture Kyoto University
-
Tanisaka Takatoshi
Graduate School Of Agriculture Kyoto Univ.
-
Okumoto Yutaka
Graduate School Of Agriculture Kyoto University
-
HAMAMOTO Yuki
Graduate School of Agriculture, Kyoto University
-
YOSHIKAKE Yoshihiro
Graduate School of Agriculture, Kyoto University
関連論文
- A genome-wide view of miniature inverted-repeat transposable elements (MITEs) in rice, Oryza sativa ssp. japonica
- Analysis of distribution and proliferation of mPing family transposons in a wild rice (Oryza rufipogon Griff.)
- Heading Time Genes Responsible for the Regional Adaptability of 'Tongil-type Short-culmed Rice Cultivars' Developed in Korea
- Gene Cloning, Bacterial Expression, and Purification of a Novel Rice (Oryza sativa L.) Ubiquitin-Related Protein, RURM1
- Application of somatic embryos to rapid and reliable analysis of soybean seed components by RNA interference-mediated gene silencing
- Ef7 Encodes an ELF3-like Protein and Promotes Rice Flowering by Negatively Regulating the Floral Repressor Gene Ghd7 under Both Short- and Long-Day Conditions
- Transgressive segregation of isoflavone contents under the control of four QTLs in a cross between distantly related soybean varieties
- Heterologous Expression of Corn Cystatin in Soybean and Effect on Growth of the Stink Bug
- Transient Dual-Luciferase Assay Combined with a Glucocorticoid-Inducible System for Rice Protoplasts