Gene Cloning, Bacterial Expression, and Purification of a Novel Rice (Oryza sativa L.) Ubiquitin-Related Protein, RURM1
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概要
- 論文の詳細を見る
A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 °C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.
- 社団法人 日本農芸化学会の論文
- 2010-02-23
著者
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Teraishi Masayoshi
Lab. Of Plant Breeding Div. Of Agronomy And Horticultural Sci. Graduate School Of Agriculture Kyoto
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Okumoto Yutaka
Graduate School of Agriculture, Kyoto University
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Okumoto Yutaka
Laboratory Of Plant Breeding Division Of Agronomy And Horticultural Science Graduate School Of Agric
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Teranishi Takeshi
Laboratory Of Plant Breeding Division Of Agronomy And Horticultural Science Graduate School Of Agric
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Teraishi Masayoshi
Laboratory Of Plant Breeding Division Of Agronomy And Horticultural Science Graduate School Of Agric
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Inouye Kuniyo
Laboratory Of Enzyme Chemistry Division Of Food Science And Biotechnology Graduate School Of Agricul
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Lee Jongwon
Laboratory Of Enzyme Chemistry Division Of Food Science And Biotechnology Graduate School Of Agricul
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TSUKIYAMA Takuji
Laboratory of Plant Breeding, Division of Agronomy and Horticultural Science, Graduate School of Agr
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TANISAKA Takatoshi
Laboratory of Plant Breeding, Division of Agronomy and Horticultural Science, Graduate School of Agr
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Tanisaka Takatoshi
Laboratory Of Plant Breeding Faculty Of Agriculture Kyoto University
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Tanisaka Takatoshi
Graduate School Of Agriculture Kyoto Univ.
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