Stimulation and Inhibition of Arabidopsis Seed Germination with Repetitive Barrier Discharges Produced by Polarity-Reversed Voltage Pulses

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This paper presents an experimental study on control of germination for Arabidopsis seed with a barrier discharge produced by polarity-reversed voltage pulses. 100 ns-width polarity-reversed pulses are applied to the seeds between plane electrodes covered by glass barriers. The electrode configuration allows the barrier discharge propagation along the seed surface at a relatively low voltage. After the discharge treatment, the seeds are incubated for 2 or 3 days to measure the germination rate. Relationships between the cumulative input energy density into plasma and the germination rate are investigated. Germination rate is significantly reduced by 20 J/cm3. However, it increases when the cumulative energy density is up to 50 J/cm3 and then it decreases again by the further energy input. These results propose a superposition of two effects, stimulation and inhibition, of the discharge treatment on the germination of Arabidopsis seed. Results of evans-blue dyeing for the discharge-exposed seeds indicate that necrosis of the seed coat is caused by the discharge treatment.