QTL Mapping of Genes Controlling Plasma Insulin and Leptin Concentrations: Metabolic Effect of Obesity QTLs Identified in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Inbred Mice

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概要

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• DDD.Cg-Ay female mice developed massive obesity as compared with B6.Cg-Ay female mice. We previously identified quantitative trait loci (QTLs) for obesity on chromosomes 1, 6, 9 and 17 in F2 female mice, including F2 Ay (F2 mice with the Ay allele) and F2 non-Ay mice (F2 mice without the Ay allele), produced by crossing C57BL/6J and DDD.Cg-Ay strains. We here addressed the question whether the obesity QTLs share genetic bases with putative QTLs for plasma glucose, insulin and leptin concentrations. We performed QTL analyses for the first principal component (PC1) extracted from these metabolic measurements to identify the genes that contributed to the comprehensive evaluation of metabolic traits. By single QTL scans, we identified two significant QTLs for insulin concentration on chromosomes 6 and 12, three for leptin concentration on chromosomes 1, 6 and 17, and five for PC1 on chromosomes 1, 6, 12 (two loci) and 17. Although insulin and leptin concentrations and PC1 were not normally distributed in combined F2 mice, results of single QTL scans by parametric and non-parametric methods were very similar. Therefore, QTL scan by the parametric method was performed with the agouti locus genotype as a covariate. A significant QTL × covariate interaction was found for PC1 on chromosome 9. All obesity QTLs had significant metabolic effects. Thus, obesity- and diabetes-related traits in DDD.Cg-Ay mice were largely controlled by QTLs on chromosomes 1, 6, 9, 12 and 17.

著者

• Suto Jun-ichi

  Agrogenomics Res. Center National Inst. Of Agrobiological Sciences

• SUTO Jun-ichi

  Agrogenomics Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305–8634, Japan

関連論文

• Quantitative Trait Loci that Control Body Weight and Obesity in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Mice
• Identification of the Modifier Locus that Suppresses Neonatal Lethality in (♀DDD × ♂DH-Dh/+) F1-Dh/+ Male Mice
• Quantitative Trait Loci that Control Plasma Lipid Levels in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Inbred Mouse Strains
• Confirmation of sable QTL that modifies the effects of the Ay allele on yellow coat color on mouse chromosome 1
• Development of an Efficient Method for Genotyping at the gd Locus in NC/Sgn Mice Based on PCR-RFLP Analysis
• QTL Mapping of Genes Controlling Plasma Insulin and Leptin Concentrations: Metabolic Effect of Obesity QTLs Identified in an F2 Intercross between C57BL/6J and DDD.Cg-Ay Inbred Mice

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