Comparative Evaluation of Three Commercial ELISA Kits for Detection of Antibodies to a Nonstructural Protein of Foot-and-Mouth Disease Virus

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概要

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• In this study, we validated three commercial ELISA (NSP-ELISA) kits that detect antibodies to a nonstructural protein of foot-and-mouth disease virus (FMDV) in terms of their specificities and sensitivities. Although the specificities of the NSP-ELISA kits were as high as that of liquid-phase blocking ELISA (LPBE) in non-infected, non-vaccinated animals, the sensitivities of the NSP-ELISA kits were significantly lower than those of the present LPBE and did not agree with the findings of a previous report on infected animals in the field. Therefore, although countries can adopt both a "vaccination-to-kill" policy and a "vaccination-to-live" policy after emergency vaccination during an FMD epidemic, the NSP-ELISA kits do not seem to be suitable for the latter policy in Japan. These results should be useful for choosing appropriate control measures for potential future FMD epidemics in Japan and elsewhere.

• 公益社団法人 日本獣医学会の論文

著者

• YOSHIDA Kazuo

  Exotic Diseases Research Station, National Institute of Animal Health

• Fukai Katsuhiko

  Exotic Diseases Research Station National Institute Of Animal Health

• MORIOKA Kazuki

  Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan

• SAKAMOTO Kenichi

  Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan

• ONOZATO Hiroyuki

  Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan

関連論文

• Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D(Virology)
• An Experimental Infection in Pigs Using a Foot-and-Mouth Disease Virus Isolated from the 2010 Epidemic in Japan
• Design of Chicken Lysozyme Signal Peptide for the Effective Secretion of Recombinant Protein in Eukaryotes
• Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D
• Comparative Evaluation of Three Commercial ELISA Kits for Detection of Antibodies to a Nonstructural Protein of Foot-and-Mouth Disease Virus

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