Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D(Virology)
スポンサーリンク
概要
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Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural proteins 2C and 3D were used as the antigens in a western blotting assay. This assay allowed for differentiation of FMDV-infected pigs from vaccinated pigs. Serial studies were performed using sera collected from experimentally infected and vaccinated pigs. Positive reactions were found in the sera derived from the experimentally infected pigs. There was, however, no positive reaction in the vaccinated pigs. These results suggest that this western blotting assay is a useful method for differentiation of infected pigs from vaccinated pigs.
- 2008-12-25
著者
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FUKAI Katsuhiko
Exotic Diseases Research Station, National Institute of Animal Health
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MORIOKA Kazuki
Exotic Diseases Research Station, National Institute of Animal Health
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OHASHI Seiichi
Exotic Diseases Research Station, National Institute of Animal Health
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YAMAZOE Reiko
Exotic Diseases Research Station, National Institute of Animal Health
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YOSHIDA Kazuo
Exotic Diseases Research Station, National Institute of Animal Health
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SAKAMOTO Kenichi
Exotic Diseases Research Station, National Institute of Animal Health
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Yamazoe Reiko
Exotic Diseases Research Station National Institute Of Animal Health
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Morioka Kazuki
Exotic Diseases Research Station National Institute Of Animal Health
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Ohashi Seiichi
Exotic Diseases Research Station National Institute Of Animal Health
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Fukai Katsuhiko
Exotic Diseases Research Station National Institute Of Animal Health
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Sakamoto Kenichi
National Inst. Animal Health Tokyo Jpn
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Sakamoto Kenichi
Exotic Diseases Research Station National Institute Of Animal Health
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MORIOKA Kazuki
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
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SAKAMOTO Kenichi
Exotic Disease Research Station, National Institute of Animal Health, National Agriculture and Food Research Organization, Tokyo 187–0022, Japan
関連論文
- Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D(Virology)
- An Experimental Infection in Pigs Using a Foot-and-Mouth Disease Virus Isolated from the 2010 Epidemic in Japan
- Design of Chicken Lysozyme Signal Peptide for the Effective Secretion of Recombinant Protein in Eukaryotes
- Differentiation of Foot-and-Mouth Disease Virus-Infected Pigs from Vaccinated Pigs using a Western Blotting Assay Based on Baculovirus-Expressed Nonstructural Proteins 2C and 3D
- Comparative Evaluation of Three Commercial ELISA Kits for Detection of Antibodies to a Nonstructural Protein of Foot-and-Mouth Disease Virus