Mandelate Racemase and Mandelate Dehydrogenase Coexpressed Recombinant Escherichia coli in the Synthesis of Benzoylformate
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概要
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Benzoylformate is used widely as an organic synthetic intermediate. Mandelate racemase and mandelate dehydrogenase are two enzymes used in producing benzoylformate from racemic mandelate in microbial metabolism. In the present work, the genes encoding mandelate racemase and mandelate dehydrogenase from Pseudomanas aeruginosa strain NUST were cloned and expressed in Escherichia coli to convert racemic mandelate to benzoylformate. The use of whole resting cells of recombinant E. coli allowed the conversion of 65.7 mM solution of racemic mandelate to benzoylformate at a yield of 98.9% in 45 h. The process is a promising alternative for the production of benzoylformate.
著者
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JIANG Lei
Department of Applied Chemistry, Faculty of Engineering, University of Tokyo
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JIANG Lei
Department of Bioengineering, Nanjing University of Science and Technology
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FENG Jing
Department of Bioengineering, Nanjing University of Science and Technology
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ZENG Zhen
Department of Bioengineering, Nanjing University of Science and Technology
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WANG Peng
Department of Bioengineering, Nanjing University of Science and Technology
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LI Dali
Department of Bioengineering, Nanjing University of Science and Technology
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YANG Junfang
Department of Bioengineering, Nanjing University of Science and Technology
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YANG Chengli
Department of Bioengineering, Nanjing University of Science and Technology
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