Post-death Cloning of Endangered Jeju Black Cattle (Korean Native Cattle): Fertility and Serum Chemistry in a Cloned Bull and Cow and Their Offspring

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概要

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• To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.

著者

• KIM Eun

  Mirae Biotech/Jeju National University Stem Cell Research Center

• PARK Hyo

  Mirae Biotech/Jeju National University Stem Cell Research Center

• PARK Se

  Mirae Biotech/Jeju National University Stem Cell Research Center

• Riu Key

  Faculty Of Biotechnology College Of Applied Life Sciences Jeju National University

• Kim Young

  Institute of Medical Sciences, School of Medicine, Jeju National University

• OH Chang

  Jeju National University Stem Cell Research Center, Seoul 143-854, Korea

• SONG Dong

  Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 690-756, Korea

• PARK Min

  Mirae Biotech, Seoul 143-854, Korea

• LEE Seung

  Jeju National University Stem Cell Research Center, Seoul 143-854, Korea

• CHOI Hyun

  Jeju National University Stem Cell Research Center, Seoul 143-854, Korea

• MOON Jeremiah

  Jeju National University Stem Cell Research Center, Seoul 143-854, Korea

• MUN Seong

  Jeju National University Stem Cell Research Center, Seoul 143-854, Korea

• KO Moon

  National Institute of Subtropical Agriculture, Jeju 690-150, Korea

• LEE Dong

  Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 690-756, Korea

• PARK Hyo

  Mirae Biotech, Seoul 143-854, Korea

• RIU Key

  Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 690-756, Korea

• KIM Young

  Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University

• PARK Se

  Mirae Biotech, Seoul 143-854, Korea

• KIM Young

  Institute for Livestock Promotion, Jeju Special Self-Governing Province, Jeju 690-180, Korea

• KIM Eun

  Mirae Biotech, Seoul 143-854, Korea

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