In Vitro Fertilization and Development of Porcine Oocytes Matured in Follicular Fluid
スポンサーリンク
概要
- 論文の詳細を見る
This study was conducted to assess the fertilization and development of porcine oocytes matured in a solo follicular fluid (pFF) using different in vitro culture systems and insemination periods. Cumulus-oocyte complexes (COCs), follicular cells (FCs), and pFF were collected from the follicles of ovaries. The pFF was used as a maturation medium (MpFF) after supplementation with follicle stimulating hormone (FSH) and antibiotics. The COCs were matured in a 15 ml test tube containing 3.5 ml of MpFF with FCs (5.2 × 106 cells/ml; rotating culture system) or 2 ml of MpFF without FCs in a 35-mm petri dish (static culture system) for 44 to 48 h. After maturation culture, oocytes were co-incubated with frozen-thawed spermatozoa for 5 h and then cultured for 7 days. The total mean rates of sperm penetration, normal fertilization, male pronucleus (MPN) formation, cleavage, and development to the blastocyst stage of oocytes after insemination were significantly higher (P<0.01) in the rotating culture system than in the static culture system. In conclusion, compared with the static culture system, the rotating culture system is adequate for the production of developmentally competent porcine oocytes when MpFF is used as a maturation medium.
著者
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ONISHI Akira
Transgenic Animal Research Center, National Institute of Agrobiological Sciences
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Fuchimoto Dai-ichiro
Transgenic Animal Research Center National Institute Of Agrobiological Sciences
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Otoi Takeshige
The United Graduate School Of Veterinary Science Yamaguchi University
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NAGAI Takashi
NARO Institute of Livestock and Grassland Science
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OTOI Takeshige
The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8511, Japan
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AGUNG Budiyanto
NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
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SENBON Shoichiro
Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
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FUCHIMOTO Dai-ichiro
Transgenic Pig Research Unit, National Institute of Agrobiological Sciences, Ibaraki 305-0901, Japan
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