Effects of Preservation of Porcine Oocytes by Dibutyryl Cyclic AMP on in vitro Maturation, Fertilization and Development

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In this study, the effects of dibutyryl cyclic AMP (dbcAMP) on the preservation of porcine oocytes were investigated. Oocytes were preserved for 24 h or 48 h in modified NCSU37 supplemented with or without 1 mM dbcAMP. After the preservation, 98.3% and 95.2% of oocytes incubated with dbcAMP for 24 h and 48 h, respectively, were at the germinal vesicle stage, whereas about a half of the oocytes incubated without dbcAMP underwent germinal vesicle break down. These preserved oocytes were cultured for 46 h. The <I>in vitro</I> maturation (IVM) rates of oocytes preserved with dbcAMP for 24 h (84.0%) and 48 h (55.3%) were significantly higher than those of oocytes preserved without dbcAMP (48.0% and 21.6%, respectively). IVM oocytes with a visible first polar body were selected, fertilized <I>in vitro</I> (IVF), and cultured for five days. The rate of oocytes preserved for 24 h with dbcAMP and developed to the blastocyst stage after IVF was 21.4% and did not differ from that of oocytes without preservation (23.8%); however, the blastocyst development rate significantly decreased for oocytes preserved with dbcAMP for 48 h (5.2%). These results indicate that dbcAMP is effective for the preservation of porcine oocytes for 24 h without decreasing their developmental ability after IVF.
独立行政法人　国際農林水産業研究センターの論文