Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig

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概要

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• The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element.

著者

• Kim Heui-soo

  Department Of Cellular And Molecular Biology Primate Research Institute Kyoto University

• Im Gi-sun

  Animal Biotechnology Division National Institute Of Animal Science

• KIM Yun-Ji

  Department of Biological Sciences, College of Natural Sciences, Pusan National University

• Oh Keon-Bong

  Animal Biotechnology Division, National Livestock Research Institute

• Jung Yi-Deun

  Department of Biological Sciences, College of Natural Sciences, Pusan National University

• Lee Ja-Rang

  Department of Biological Sciences, College of Natural Sciences, Pusan National University

• Ha Hong-Seok

  Department of Biological Sciences, College of Natural Sciences, Pusan National University

• Choi Bong-Hwan

  Animal Genomics & Bioinformatics Division, National Institute of Animal Science

• Choi Bong-Hwan

  Animal Genomics & Bioinformatics Division, National Institute of Animal Science

• Kim Heui-Soo

  Department of Biological Sciences, College of Natural Sciences, Pusan National University

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